| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.108249 |
| Chromosome: | chromosome 6 |
| Location: | 1742139 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261800 | ADT1,PRD1 | (1 of 1) 4.2.1.91 - Arogenate dehydratase / Carboxycyclohexadienyl dehydratase; Prephenate dehydratase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAACCGGAGGTTCCCCTCGGCGCGGCGGC |
| Internal bar code: | CTGTGCTTTGACTTGATCTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 205 |
| LEAP-Seq percent confirming: | 99.7227 |
| LEAP-Seq n confirming: | 3956 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGAATGCAAGACGAACAC |
| Suggested primer 2: | CAGTAGCAGCAGCAATAGCG |