Insertion junction: LMJ.RY0402.108254_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.108254
Chromosome:chromosome 1
Location:1421956
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g007150 (1 of 1) PTHR12864:SF14 - RAN-BINDING PROTEINS 9/10 HOMOLOG intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTTGAATGGAAATGTACCAGCGTCAGGAT
Internal bar code:CGATCGTAGTAGGCGACCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:85
LEAP-Seq percent confirming:76.9231
LEAP-Seq n confirming:140
LEAP-Seq n nonconfirming:42
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACCACTGTGATCATGCGTGT
Suggested primer 2:CACGTGTTTTGATCGTGTCC