| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.108264 |
| Chromosome: | chromosome 6 |
| Location: | 1421008 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g259100 | POB30 | Proteome of basal body 30 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGAGGATCAGGTGGCCAAGGCGCGTGA |
| Internal bar code: | CGCGAGGATTTAGGCCTGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 324 |
| LEAP-Seq percent confirming: | 98.8423 |
| LEAP-Seq n confirming: | 8965 |
| LEAP-Seq n nonconfirming: | 105 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCGCAAACTCACAATTC |
| Suggested primer 2: | TCTCCCTCCCCCTCCTATTA |