| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.108284 |
| Chromosome: | chromosome 1 |
| Location: | 6180008 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044250 | CAM4 | Calmodulin-like protein; (1 of 8) 2.4.1.5 - Dextransucrase / Sucrose 6-glucosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTGGGTTAGGGGCTCGCGTCGCCTCAG |
| Internal bar code: | CTTTACGATATTTTAATCCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1133 |
| LEAP-Seq percent confirming: | 99.5238 |
| LEAP-Seq n confirming: | 7732 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAAGCAGCAGTAGCAGCA |
| Suggested primer 2: | GCTAGTGGTGGCAAATGGAT |