Insertion junction: LMJ.RY0402.108298_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.108298
Chromosome:chromosome 5
Location:2937467
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g238260 (1 of 1) PF13371//PF13414//PF13432 - Tetratricopeptide repeat (TPR_9) // TPR repeat (TPR_11) // Tetratricopeptide repeat (TPR_16) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATTCCGCCAGCGACTTGGGTGGCTGCCCC
Internal bar code:TCAGTGCGTGAAAGGGATTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:304
LEAP-Seq percent confirming:99.2573
LEAP-Seq n confirming:1470
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCACACACACACACACAC
Suggested primer 2:CCCGTTTTACGCATTTCAGT