| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.108305 |
| Chromosome: | chromosome 12 |
| Location: | 5759604 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533150 | (1 of 3) PF07452 - CHRD domain (CHRD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCCAGCACAGACCTCATGTCCCGCCGCT |
| Internal bar code: | CGTGGTTCACTGTGCACCCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 317 |
| LEAP-Seq percent confirming: | 66.9231 |
| LEAP-Seq n confirming: | 174 |
| LEAP-Seq n nonconfirming: | 86 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGTGCGGAGAGAAGGTA |
| Suggested primer 2: | CATGATGTGTGCGTGTGTGT |