Insertion junction: LMJ.RY0402.108306_4

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.108306
Chromosome:chromosome 13
Location:3741833
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTAAACCTGTCCACTGCCTGCCCTCCACAC
Internal bar code:TCGGGGACCGGCGCTTTAAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:79
LEAP-Seq percent confirming:7.14286
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTGGCGCTACAGTGACAA
Suggested primer 2:ACAAACGAAATTGTGAGCCC