| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.108312 |
| Chromosome: | chromosome 1 |
| Location: | 7257511 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g052400 | CPLD43 | CPLD43 protein, required for cyt b6 assembly | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAATGAGATCCTGCTGGGGCCGCAGGGCC |
| Internal bar code: | TATTGGCGGGTAGCAAAAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 681 |
| LEAP-Seq percent confirming: | 95.9826 |
| LEAP-Seq n confirming: | 3966 |
| LEAP-Seq n nonconfirming: | 166 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACAACCGCCTGCCTATGT |
| Suggested primer 2: | CTAACGGTCAGCCAAAGCTC |