| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.108332 |
| Chromosome: | chromosome 9 |
| Location: | 81270 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386400 | FAP124,UBA1,POC20 | (1 of 1) K03178 - ubiquitin-activating enzyme E1 (UBE1, UBA1); Ubiquitin E1 activating enzyme | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAGTGGGGAGGGGTGGGGGAAAGTCCGA |
| Internal bar code: | GCGGGCGTGGCAGTGTGAGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 562 |
| LEAP-Seq percent confirming: | 98.882 |
| LEAP-Seq n confirming: | 796 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACATCTTCCCCAAGCAC |
| Suggested primer 2: | TCCGCAGTTTCCTTTTTGAC |