| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.108332 |
| Chromosome: | chromosome_9 |
| Location: | 81271 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g386400 | FAP124,UBA1 | Ubiquitin-activating enzyme E1 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATTAAGTGTAGGATTGCAAATTTGTTCCCT |
| Internal bar code: | GTCTTAACTCATAGTGTTTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 686 |
| LEAP-Seq percent confirming: | 99.7209 |
| LEAP-Seq n confirming: | 5002 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACATCTTCCCCAAGCAC |
| Suggested primer 2: | TCCGCAGTTTCCTTTTTGAC |