| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.108358 |
| Chromosome: | chromosome 3 |
| Location: | 6658394 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197150 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCACCTTTTCTTGTCCACGCACCGCTCA |
| Internal bar code: | TGGATGCCTCTACGTTACATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 99.6673 |
| LEAP-Seq n confirming: | 3295 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACTGCACTGGCTTTTCA |
| Suggested primer 2: | GAGGTTACAAGGACGGGACA |