| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.108421 |
| Chromosome: | chromosome 16 |
| Location: | 5573395 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680100 | (1 of 1) 2.1.1.177 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase / rRNA large subunit methyltransferase H | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACCAATCACTCATCATGCACATATGCAG |
| Internal bar code: | GAGATTGAAGGGCATTTGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 99.8375 |
| LEAP-Seq n confirming: | 7371 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGATTGCTGAGACAGGCA |
| Suggested primer 2: | GTGCTTGCACGATTGCTTTA |