Insertion junction: LMJ.RY0402.108439_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.108439
Chromosome:chromosome 6
Location:9003420
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g311900 RABG1,RAB7 RAB-Like GTP binding Protein; (1 of 1) K07897 - Ras-related protein Rab-7A (RAB7A) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTGCTGGGACCCCGTCGCTCCTGTTCCA
Internal bar code:TCGGATCTCCCATTGTTTTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:637
LEAP-Seq percent confirming:99.8291
LEAP-Seq n confirming:584
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CACATGATGACACGACCACA
Suggested primer 2:GACATGAACACCACTGCCAC