| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.108439 |
| Chromosome: | chromosome 6 |
| Location: | 9003428 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311900 | RABG1,RAB7 | RAB-Like GTP binding Protein; (1 of 1) K07897 - Ras-related protein Rab-7A (RAB7A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCCATTGATGGTCCGGGATGGAGATAG |
| Internal bar code: | GTTCTCCGACTGGAAGGGCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 853 |
| LEAP-Seq percent confirming: | 99.5077 |
| LEAP-Seq n confirming: | 2830 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACATGATGACACGACCACA |
| Suggested primer 2: | GACATGAACACCACTGCCAC |