| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.108465 |
| Chromosome: | chromosome 7 |
| Location: | 2855054 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g332100 | (1 of 2) 1.3.3.5 - Bilirubin oxidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTTCACTATCCTTAGTCCCATACGATGC |
| Internal bar code: | CCCACGCTCCCTTACTGGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 688 |
| LEAP-Seq percent confirming: | 99.6296 |
| LEAP-Seq n confirming: | 538 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAAATCGCTCAGGAATGG |
| Suggested primer 2: | TAGCATACGCCCCTGTTACC |