| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.108529 |
| Chromosome: | chromosome_2 |
| Location: | 6292057 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g114600 | PRX2 | 2-cys peroxiredoxin | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACGTCAGAGTGTCACAAACCTTCCATCGCA |
| Internal bar code: | GAGCGCTTTTTTCATTCCCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 209 |
| LEAP-Seq percent confirming: | 4.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACGCATTTTTGGGCTGTC |
| Suggested primer 2: | AAGATACCGCGTGTTGAACC |