Insertion junction: LMJ.RY0402.108533_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.108533
Chromosome:chromosome 5
Location:2674648
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g236550 (1 of 41) PTHR10157 - DOPAMINE BETA HYDROXYLASE RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCGCGCCCGTCCCAGCACTTCCACACGC
Internal bar code:CACTGCTGTGGTCCATCCACGT

Confirmation - LEAP-Seq

LEAP-Seq distance:114
LEAP-Seq percent confirming:68.1529
LEAP-Seq n confirming:107
LEAP-Seq n nonconfirming:50
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGCCACCAACTGCTACTCTG
Suggested primer 2:GTGTGACACGTTCTTCCCCT