| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.108542 |
| Chromosome: | chromosome 12 |
| Location: | 4245546 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g519150 | (1 of 1) K12169 - Kip1 ubiquitination-promoting complex protein 1 [EC:6.3.2.19] (KPC1, RNF123) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGACCATACCCTAGGAAGCGCCCTTCCGT |
| Internal bar code: | GCTTGGCTGTGAGTAATTGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 38 |
| LEAP-Seq percent confirming: | 67.4157 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGCTGCTGAGATGTTCT |
| Suggested primer 2: | TCCCCACTACGGCAATAGAC |