| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.108572 |
| Chromosome: | chromosome 9 |
| Location: | 1952937 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394900 | CYA10 | (1 of 1) IPR000209//IPR001054//IPR029787 - Peptidase S8/S53 domain // Adenylyl cyclase class-3/4/guanylyl cyclase // Nucleotide cyclase; Solute-binding protein-like adenylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTGTCGGGTCTGCGCAGCGGCAGCGCA |
| Internal bar code: | CCATAACCTGCAAGGTGTTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 110 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 191 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCGGAAACGTGGACAGAG |
| Suggested primer 2: | ACTTGCTGCCAACAGGAAAC |