| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.108578 |
| Chromosome: | chromosome 8 |
| Location: | 4485556 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g382515 | (1 of 1) PTHR22838//PTHR22838:SF0 - WD REPEAT PROTEIN 26-RELATED // WD REPEAT-CONTAINING PROTEIN 26 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGGATGAGCAGCACGGTTTTTTTTCCC |
| Internal bar code: | TCGACCTGGGCTCCCTCCGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 594 |
| LEAP-Seq percent confirming: | 99.2657 |
| LEAP-Seq n confirming: | 9598 |
| LEAP-Seq n nonconfirming: | 71 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGCAGCAATACAAAGCAA |
| Suggested primer 2: | AACACGCTTCACCTTGCTTT |