| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.108583 |
| Chromosome: | chromosome 10 |
| Location: | 95237 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418300 | (1 of 30) PF03407 - Nucleotide-diphospho-sugar transferase (Nucleotid_trans) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGCTGTGCGGAGTAAGCAGTAAATGGC |
| Internal bar code: | TTCCCTCAGGGCCTTCGGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 396 |
| LEAP-Seq percent confirming: | 99.8243 |
| LEAP-Seq n confirming: | 2841 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCTACGAGCTTTTGCACC |
| Suggested primer 2: | CAGAGGGCTGAAACTCAAGG |