| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.108642 |
| Chromosome: | chromosome 3 |
| Location: | 4291845 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174600 | (1 of 3) PF13771 - PHD-like zinc-binding domain (zf-HC5HC2H) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGAAACCATGGCGGACCAACACGCGTG |
| Internal bar code: | AGGGAGAGTAAAAGATGGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 313 |
| LEAP-Seq percent confirming: | 90.7721 |
| LEAP-Seq n confirming: | 1446 |
| LEAP-Seq n nonconfirming: | 147 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCAGTCGATATCAGTCAA |
| Suggested primer 2: | GGAGCTAGAGAGCATCACGG |