| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.108671 |
| Chromosome: | chromosome_9 |
| Location: | 3097278 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g386850 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGTTAACCCCCTCTCCCCACACGCCTGTAC |
| Internal bar code: | GAGTTGTTATCGGGGTATCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 480 |
| LEAP-Seq percent confirming: | 98.1919 |
| LEAP-Seq n confirming: | 1412 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCACTCTGTTTCACACAT |
| Suggested primer 2: | GCTCACGGTTTCAGCTTTTC |