| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.108696 |
| Chromosome: | chromosome 6 |
| Location: | 5089234 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280750 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTCGGGAGGGGGCGGCGGGCTGTCAAC |
| Internal bar code: | GCGAACTGTAGAATCAAATATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1030 |
| LEAP-Seq percent confirming: | 99.8175 |
| LEAP-Seq n confirming: | 6562 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCAGCAGAGCGAGGACAT |
| Suggested primer 2: | TCGCAATTTCCCCAACTATC |