| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.108708 |
| Chromosome: | chromosome_11 |
| Location: | 2623065 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g476376 | FAP221 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGTCCAGGCTGCCGTACGGGCTGTACGACG |
| Internal bar code: | ATATGTGTGCTTAAACCCAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 471 |
| LEAP-Seq percent confirming: | 99.7195 |
| LEAP-Seq n confirming: | 6399 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCAGCCCAAACTGAACG |
| Suggested primer 2: | TAGCGGTAAAGGAGGGGTTT |