Insertion junction: LMJ.RY0402.108708_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.108708
Chromosome:chromosome 11
Location:2623081
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g476376 FAP221,Pcdp1,RPC82 Flagellar Associated Protein 221; (1 of 1) PTHR23053//PTHR23053:SF21 - DLEC1 DELETED IN LUNG AND ESOPHAGEAL CANCER 1 // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGCGGCAGTACGACGTGTACGACAGCGC
Internal bar code:GCTATAGGACCGACGGTGGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:371
LEAP-Seq percent confirming:93.8775
LEAP-Seq n confirming:138
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ATACCAGCCCAAACTGAACG
Suggested primer 2:CGACTGGAGGTGGGTAGGTA