| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.108708 |
| Chromosome: | chromosome_11 |
| Location: | 2623081 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g476376 | FAP221 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCGCGGCAGTACGACGTGTACGACAGCGC |
| Internal bar code: | GCTATAGGACCGACGGTGGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 371 |
| LEAP-Seq percent confirming: | 93.8775 |
| LEAP-Seq n confirming: | 138 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCAGCCCAAACTGAACG |
| Suggested primer 2: | CGACTGGAGGTGGGTAGGTA |