| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.108723 |
| Chromosome: | chromosome 2 |
| Location: | 5269512 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g106450 | LCA1 | (1 of 1) PTHR11440//PTHR11440:SF4//PTHR23070//PTHR23070:SF14 - LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED // PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE // BCS1 AAA-TYPE ATPASE // SUBFAMILY NOT NAMED; AAA ATPase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCGCCGCCGGTGCGGGTGTGGCAGGGG |
| Internal bar code: | CCTCCCGGGACTGTCTGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 448 |
| LEAP-Seq percent confirming: | 95.2653 |
| LEAP-Seq n confirming: | 1167 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTGGGGACGTAGAGGTA |
| Suggested primer 2: | AAGCAGACAAGCACCAACCT |