| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.108732 |
| Chromosome: | chromosome 11 |
| Location: | 3169798 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479350 | (1 of 3) PTHR31563//PTHR31563:SF1 - FAMILY NOT NAMED // ION CHANNEL POLLUX-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAGCACTGGTGCCGCCAACGGCCGTGCC |
| Internal bar code: | GTGGAAATCGTTGCAGAGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 508 |
| LEAP-Seq percent confirming: | 95.8545 |
| LEAP-Seq n confirming: | 1133 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAACTTGGGTTGTCCATA |
| Suggested primer 2: | GTGGAGCCACTGGTATTGCT |