| Insertion cassette: | CIB1 | 
| Side of cassette: | 3' | 
| Strand: | + | 
| Strain: | LMJ.RY0402.108743 | 
| Chromosome: | chromosome 12 | 
| Location: | 5223552 | 
| Confidence (%): | 95 | 
| Locus systematic id | Locus common name | Defline | Feature | 
|---|---|---|---|
| Cre12.g528100 | CPZ | Zinc carboxypeptidase; (1 of 1) PTHR12756:SF12 - CYTOSOLIC CARBOXYPEPTIDASE-LIKE PROTEIN 5 | intron | 
| Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTTCTCACATCGAATGACACGGACAGG | 
| Internal bar code: | GAATTGCATGTGAGAAAAATGA | 
| Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 344 | 
| LEAP-Seq percent confirming: | 97.249 | 
| LEAP-Seq n confirming: | 707 | 
| LEAP-Seq n nonconfirming: | 20 | 
| LEAP-Seq n unique pos: | 3 | 
| Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCAAAATCATACCACGC | 
| Suggested primer 2: | ACTTTGACCATGATGCCTCC |