| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.108751 |
| Chromosome: | chromosome 6 |
| Location: | 3485269 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278099 | (1 of 2) IPR003382 - Flavoprotein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGCAAAAAACAGGCCCGACTCCGGTTCC |
| Internal bar code: | TTTTCCCGGGGGCCTCCGTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1160 |
| LEAP-Seq percent confirming: | 99.6054 |
| LEAP-Seq n confirming: | 8834 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTACCTTCTGGACAGCGAG |
| Suggested primer 2: | ATCCCGACCTATTTATGGGC |