| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.108848 |
| Chromosome: | chromosome 12 |
| Location: | 1053920 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g491000 | (1 of 14) PF04577 - Protein of unknown function (DUF563) (DUF563) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTAAGCACTGAACCCCTCAACCTTGCAC |
| Internal bar code: | CGGACTACTTCCCTTGCAGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 682 |
| LEAP-Seq percent confirming: | 99.6898 |
| LEAP-Seq n confirming: | 2571 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGGTAACCCGTCCGTAT |
| Suggested primer 2: | ATCTGGCAGAATAACCGTGG |