| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.108866 |
| Chromosome: | chromosome 17 |
| Location: | 5394107 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g736650 | (1 of 2) K01256 - aminopeptidase N (pepN) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATGTAGCTGTCAGGGCGGACCGGGTGG |
| Internal bar code: | GAAAACTATACCGTGAGAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 885 |
| LEAP-Seq percent confirming: | 98.7979 |
| LEAP-Seq n confirming: | 1315 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTGTAGGTCTGGCTGAT |
| Suggested primer 2: | TAGTCTGCTGGTGGTTGCTG |