| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.108875 |
| Chromosome: | chromosome 2 |
| Location: | 1748325 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g086428 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATAGCACACGGTCCCCACGGCACCATGG |
| Internal bar code: | ATAAGGCGAGCACATCTCGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 165 |
| LEAP-Seq percent confirming: | 8.05044 |
| LEAP-Seq n confirming: | 498 |
| LEAP-Seq n nonconfirming: | 5688 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACACAACATCCAGGTGC |
| Suggested primer 2: | GCCACCTCCTTGTTGTTTGT |