| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.108916 |
| Chromosome: | chromosome 5 |
| Location: | 3373351 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240900 | (1 of 1) IPR000001//IPR024079 - Kringle // Metallopeptidase, catalytic domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAAATGGAGGCGTTTGAAGATCCCAAATT |
| Internal bar code: | GCACGCGACCCGGTATAAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 690 |
| LEAP-Seq percent confirming: | 99.0862 |
| LEAP-Seq n confirming: | 2494 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTAACACCCCTCATCTCGT |
| Suggested primer 2: | CGAGGCATAGGTACGGTGAT |