Insertion junction: LMJ.RY0402.108920_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.108920
Chromosome:chromosome 2
Location:284796
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g075050 PTA1 (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain; Proton/phosphate symporter intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAACAAGGACAGGCGAAGCAGCGCACATA
Internal bar code:CACCGGAGACTGTCATTGCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:736
LEAP-Seq percent confirming:98.4375
LEAP-Seq n confirming:1638
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGGCCAGCTACATTTTGG
Suggested primer 2:CTTGAGCGCACAACTGGTAA