Insertion junction: LMJ.RY0402.108936_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.108936
Chromosome:chromosome 13
Location:4371894
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g602901 CNX2 (1 of 1) PTHR22960:SF0 - MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1; Cofactor of nitrate reductase and xanthine dehydrogenase 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTCCACTTCTTCTTCTACACACAACCCA
Internal bar code:CCCTTCCGTGCCAACCGCTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:565
LEAP-Seq percent confirming:99.2722
LEAP-Seq n confirming:682
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACCAGAGTCGTAAACGGTGC
Suggested primer 2:GAGACCTCATGCGAGAGTCC