| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.108951 |
| Chromosome: | chromosome 13 |
| Location: | 3045765 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g584450 | (1 of 6) PTHR27000:SF160 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCGGGGACCATCCCATCGGCCTGGAGC |
| Internal bar code: | CCACGTGGCGGGTGATGGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 382 |
| LEAP-Seq percent confirming: | 99.1285 |
| LEAP-Seq n confirming: | 910 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACAGCAGCATCACACAGG |
| Suggested primer 2: | CTTTGCAGGTTCAGGTTGGT |