| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.108960 |
| Chromosome: | chromosome 10 |
| Location: | 3122513 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441750 | (1 of 4) PTHR11772//PTHR11772:SF2 - ASPARAGINE SYNTHETASE // ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGCACGCTCTTGCCGCGGCTGCTAAACT |
| Internal bar code: | AGACCTCGTGGTTAGGTAACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 256 |
| LEAP-Seq percent confirming: | 99.5178 |
| LEAP-Seq n confirming: | 1032 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGGCAAGCTGTCCTTTA |
| Suggested primer 2: | GCTTGGACTCTTTGGCTCAC |