Insertion junction: LMJ.RY0402.108981_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.108981
Chromosome:chromosome 9
Location:6257273
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g406400 CTP3 (1 of 2) K17686 - Cu+-exporting ATPase (copA, ATP7); Copper transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAAGCCAACGGAAACGAGCGGAGGACAGG
Internal bar code:CACGTATAAGGTGTAGCAGTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:214
LEAP-Seq percent confirming:95.0164
LEAP-Seq n confirming:1735
LEAP-Seq n nonconfirming:91
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CACCCAATAAACACACGCAG
Suggested primer 2:CCCTGATCATCACGTTTGTG