Insertion junction: LMJ.RY0402.109007_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.109007
Chromosome:chromosome 3
Location:1775280
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g154050 ABCA1B putative ABC transporter; (1 of 173) IPR029058 - Alpha/Beta hydrolase fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGATGCGGACCGCTGAGGCCACACGAAAAC
Internal bar code:CCAACACCTCGACCCTTATGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1037
LEAP-Seq percent confirming:99.2599
LEAP-Seq n confirming:2146
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GGATGGGGAGGATAAGTGGT
Suggested primer 2:AGGAACGTGAGTTTGTTGGG