Insertion junction: LMJ.RY0402.109027_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.109027
Chromosome:chromosome 3
Location:7233247
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g207750 (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACACGACCCGTGGCCCGTTGATTAACTT
Internal bar code:TACCCATCAAATTCCGACCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:5
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTGAGTAAAGCTGCCCTTCG
Suggested primer 2:CCTGCTGTTTTCAACCCAGT