| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.109078 |
| Chromosome: | chromosome 12 |
| Location: | 5490632 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530900 | (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGCGTGAGAGATTCGGATGCCCTTGCGG |
| Internal bar code: | GCCTGGCGGCAGTAGACATCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 891 |
| LEAP-Seq percent confirming: | 99.0688 |
| LEAP-Seq n confirming: | 1915 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCCTTTGACTGAACTCG |
| Suggested primer 2: | TCACGGAAGTGTGTGTTGGT |