| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.109096 |
| Chromosome: | chromosome 13 |
| Location: | 2994465 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g584200 | GRL1 | Glutaredoxin-like protein; (1 of 1) PF03595 - Voltage-dependent anion channel (SLAC1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGTGCGGCGGCGCCAAGGGCGCAGGTGA |
| Internal bar code: | AATACCACGATGGTCTAATGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 576 |
| LEAP-Seq percent confirming: | 99.4249 |
| LEAP-Seq n confirming: | 7088 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGTAGGTTGCGGCATGGT |
| Suggested primer 2: | CTGCGAAGGTCCATAGAAGC |