| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.109108 |
| Chromosome: | chromosome 3 |
| Location: | 7996818 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201700 | (1 of 1) PF15072 - Domain of unknown function (DUF4539) (DUF4539) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGTCCTGCACCAGCAGTGTGTCGTGTAC |
| Internal bar code: | CTTGTTCTGCCGCAGTTATCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 383 |
| LEAP-Seq percent confirming: | 98.4596 |
| LEAP-Seq n confirming: | 3068 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAATAGTCGTCGGACGGT |
| Suggested primer 2: | GAGACAGTAAGCCAGCGTCC |