| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.109116 |
| Chromosome: | chromosome 1 |
| Location: | 7666987 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g055408 | ACS2 | (1 of 3) K01895 - acetyl-CoA synthetase (ACSS, acs); Acetyl-CoA synthase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTGGAGCAGCTCGGCCGCCATGCAAACA |
| Internal bar code: | AGGCCATCGTTTGTGAAACCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 627 |
| LEAP-Seq percent confirming: | 98.2948 |
| LEAP-Seq n confirming: | 3574 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCATCAACCTCCTCCAA |
| Suggested primer 2: | GAGCGGCCTTCAGTTACAAG |