| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.109133 |
| Chromosome: | chromosome 10 |
| Location: | 1090279 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425450 | AOF5 | (1 of 1) 1.5.99.4 - Nicotine dehydrogenase; Flavin-containing amine oxidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTTGCCACCACCGCCTTTCTGCGGGGAA |
| Internal bar code: | TAGGTCGAGGATCGATTGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 397 |
| LEAP-Seq percent confirming: | 99.7087 |
| LEAP-Seq n confirming: | 5818 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCATAACCTCTCTCTTGC |
| Suggested primer 2: | CGCGAACATCAAAGCACTAA |