| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.109155 |
| Chromosome: | chromosome 8 |
| Location: | 30374 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358350 | OPR34,TDA1 | Translation factor for atpA | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCGGCTGCATTCTGTCCACTGCCCTTGC |
| Internal bar code: | GGGATTCAGATCGGGTGAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 670 |
| LEAP-Seq percent confirming: | 99.4565 |
| LEAP-Seq n confirming: | 183 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCTATTCGGGTTCTGGG |
| Suggested primer 2: | TGCCGTTAATCCGTTCCTAC |