| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.109161 |
| Chromosome: | chromosome 1 |
| Location: | 4985657 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034451 | (1 of 1) K14012 - UBX domain-containing protein 1 (SHP1, UBX1, NSFL1C) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATCCACATCTGCTCCGTGCATTACCATGC |
| Internal bar code: | GTCAGGCTCAAGAATCACGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 570 |
| LEAP-Seq percent confirming: | 97.9557 |
| LEAP-Seq n confirming: | 3977 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCAGGTAGTGGTAGTGGT |
| Suggested primer 2: | CACCCTCACTTACCTGCCAT |