Insertion junction: LMJ.RY0402.109213_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.109213
Chromosome:chromosome 14
Location:1005827
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre14.g614226 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCCCACAATCCATACACGGTTGTGGTCT
Internal bar code:TCTATGGACACACGCATACCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:279
LEAP-Seq percent confirming:99.6
LEAP-Seq n confirming:747
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CAATACATGTGGGCTGCAAG
Suggested primer 2:AGTGGTAGATGAACCCGTGC