Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.109233 |
Chromosome: | chromosome_6 |
Location: | 3825023 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g278170 | FAP237,FAS7 | Fasciclin-like protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGGTCCAGTCTAATGTGCAGGGACGATGGG |
Internal bar code: | TCTCGCGGTCGAGAGGACACAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 624 |
LEAP-Seq percent confirming: | 86.1584 |
LEAP-Seq n confirming: | 1164 |
LEAP-Seq n nonconfirming: | 187 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCGGCACTGTACTAGCATGA |
Suggested primer 2: | TAGGTTGTTGGTCTGAGGGG |